The current challenge in microbiome research lies in the absence of suitable models for dissecting mechanisms and causality. Our project aims to revolutionise this landscape by crafting advanced microbiome models on human in vitro organoid platforms, with a specific emphasis on establishing a cutting-edge gut microbiome platform. This endeavour involves seamlessly integrating organ-specific cells into meticulously engineered tissue micro-environments, employing state-of-the-art biomaterials and additive manufacturing processes. By harnessing bioreactor technologies, we will construct comprehensive models, providing a sophisticated framework to delve into the intricate mechanisms and causality within microbiome research. Our mission extends further to evaluate the microbiome's susceptibility to the resistome, gauging its repercussions on ecosystem stability within these pioneering platforms.